Changes between Version 17 and Version 18 of FAQ
- Timestamp:
- 07/19/22 12:47:51 (3 years ago)
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FAQ
v17 v18 37 37 1. Why do I get '''[#wi_ncbi_blast different BLAST results]''' from [[http://tak.wi.mit.edu/blast/ | WI]] and NCBI Blast? [[br]] [[br]] 38 38 1. How do I run '''[#tophat_bowtie tophat/bowtie on the LSF with a gzip'd tar (*.tar.gz)]''' file? [[br]] [[br]] 39 1. How can I run '''[#alphafold AlphaFold 2.0 or 2.1]''' here at Whitehead? [[br]] [[br]]39 1. How can I run '''[#alphafold AlphaFold 2.0-2.2]''' here at Whitehead? [[br]] [[br]] 40 40 1. How can I output '''[#pvalues small p-values]''' from R? [[br]] [[br]] 41 41 ---- … … 209 209 * bsub bash -c "tophat ... <(tar xvzfO ...) <(tar xvzfO ...)", this is using process substitution 210 210 * eg. bsub bash -c "tophat -p 10 -g 1 -o mapped_data_SRR905147_unique -N 2 -I 10000 --segment-length 25 --segment-mismatches 2 hg19 <(tar xvzfO s_2_1_sequence.txt.tar.gz ACTTGA-s_2_1_sequence.txt) <(tar xvzfO CAGATC-s_2_1_sequence.txt.tar.gz CAGATC-s_2_1_sequence.txt)" 211 1. [=#alphafold How can I run '''AlphaFold 2.0 or 2.1''' here at Whitehead?]211 1. [=#alphafold How can I run '''AlphaFold 2.0-2.2''' here at Whitehead?] 212 212 * You'll need access to a system with a GPU, or the new GPU queue. IT can help you choose and obtain access to GPU systems if required. 213 213 * For a working command to be executed on fry.wi.mit.edu, which then sends the AlphaFold command to the GPU node on the slurm cluster, see /nfs/BaRC_Public/BaRC_code/shell/run_AlphaFold/Commands.sh … … 217 217 sbatch -J AF200_1 --export=ALL,FASTA_NAME=Sample_protein_1.fa,USERNAME=myUsername,FASTA_PATH=proteins,AF2_WORK_DIR=/nfs/BaRC_Public/BaRC_code/shell/run_AlphaFold ./RunAlphaFold_2.0.0_slurm.sh 218 218 sbatch -J AF212_1 --export=ALL,FASTA_NAME=Sample_protein_1.fa,USERNAME=myUsername,FASTA_PATH=proteins,AF2_WORK_DIR=/nfs/BaRC_Public/BaRC_code/shell/run_AlphaFold ./RunAlphaFold_2.1.2_slurm.sh 219 sbatch -J AF220_1 --export=ALL,FASTA_NAME=Sample_protein_1.fa,USERNAME=myUsername,FASTA_PATH=proteins,AF2_WORK_DIR=/nfs/BaRC_Public/BaRC_code/shell/run_AlphaFold ./RunAlphaFold_2.2.0_slurm.sh 219 220 }}} 220 221 where the inputs are