Changes between Version 20 and Version 21 of SOP/CallingVariantsRNAseq
- Timestamp:
- 08/16/17 12:36:11 (7 years ago)
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SOP/CallingVariantsRNAseq
v20 v21 33 33 }}} 34 34 35 36 37 38 39 35 The parameters included in the above sample commands are: 40 36 * '''--runMode <alignReads, genomeGenerate>''' "alignReads" does the actual mapping. "genomeGenerate" generates the genomeDir required for mapping (default = alignReads). … … 42 38 * '''--genomeFastaFiles <genome FASTA files>''' Specifies genome FASTA files to be used. 43 39 * '''--readFilesIn <read1.fastq read2.fastq> ''' Specifies the fastq files containing the reads, can be single-end or paired-end. 44 * '''--sjdbScore <n> ''' Provides extra alignment score for alignments that cross database junctions (default = 2). If this score is positive, it will bias the alignment toward annotated junctions. This is only used if during the genomeGenerate step a splice junction annotation file is used.45 40 * '''--runThreadN <n> ''' Specifies the number of threads to use.