Changes between Version 36 and Version 37 of SOP/CallingVariantsRNAseq
- Timestamp:
- 08/16/17 13:38:30 (7 years ago)
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SOP/CallingVariantsRNAseq
v36 v37 65 65 '''2 - Replace ReadGroups, mark duplicate reads , clip intron overhangs and reassign mapping qualities with[http://broadinstitute.github.io/picard/ Picard Tools]''' 66 66 67 67 {{{java -jar /usr/local/share/picard-tools/picard.jar AddOrReplaceReadGroups I=output.sam O=rg_added_sorted.bam SO=coordinate RGID=ID_NAME RGLB=library RGPL=illumina RGPU=identifier RGSM=sample_name}}}