Changes between Version 62 and Version 63 of SOP/CallingVariantsRNAseq
- Timestamp:
- 09/06/17 09:02:09 (7 years ago)
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SOP/CallingVariantsRNAseq
v62 v63 9 9 == Using GATK to call variants from RNA-seq reads == 10 10 This example pipeline starts with a single-end short-read fastq file (Reads_1.fq). 11 \\ \\12 Note that '''GATK requires Java 1.8''(so you may need to adjust your path to point to that version, if an older version is the default).\\11 \\ 12 Note that GATK requires Java 1.8 (so you may need to adjust your path to point to that version, if an older version is the default).\\ 13 13 For example, this can be added to ~/.bashrc: \\ 14 14 export PATH=/path/to/java:$PATH 15 \\ \\ 16 15 \\ 17 16 18 17 '''1 - Run the STAR 2-pass procedure to map reads to the reference genome.'''