Changes between Version 64 and Version 65 of SOP/CallingVariantsRNAseq
- Timestamp:
- 09/06/17 10:08:00 (7 years ago)
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SOP/CallingVariantsRNAseq
v64 v65 16 16 17 17 18 '''1 - Run the STAR 2-pass procedure to map reads to the reference genome.''' 19 20 For the STAR 2-pass mapping procedure, please see our mapping SOP, under STAR. 18 '''1 - Run the STAR 2-pass procedure to map reads to the reference genome'''. 19 For the STAR 2-pass mapping procedure, please see our mapping SOP, under [http://barcwiki.wi.mit.edu/wiki/SOPs/mapping STAR]. 21 20 22 21 '''2 - Replace ReadGroups, mark duplicate reads , clip intron overhangs and reassign mapping qualities with[http://broadinstitute.github.io/picard/ Picard Tools] and [https://software.broadinstitute.org/gatk/download/ GATK]'''