Changes between Version 4 and Version 5 of SOP/Demultiplex_HTO_ADT_data


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Timestamp:
05/26/21 13:33:50 (3 years ago)
Author:
ibarrasa
Comment:

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  • SOP/Demultiplex_HTO_ADT_data

    v4 v5  
    11== Demultiplexing hashtag oligos (HTOs) ==
    22=== Demultiplexing with hashtag oligos (HTOs) and analysis with Seurat ===
    3 Follow the the Seurat vignette on hashing[[https://satijalab.org/seurat/archive/v3.1/hashing_vignette.html]], but on the demultiplex step use kfunc = “kmeans”.
     3Follow the the Seurat v3 vignette on hashing[[https://satijalab.org/seurat/archive/v3.1/hashing_vignette.html]], but on the demultiplex step use kfunc = “kmeans”.
    44These are the main steps:
    55