Changes between Version 16 and Version 17 of SOP/PooledCRISPR


Ignore:
Timestamp:
11/23/21 15:47:43 (3 years ago)
Author:
thiruvil
Comment:

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  • SOP/PooledCRISPR

    v16 v17  
    7373== Method 2: MAGeCK ==
    7474
    75 Analyze CRISPR genome-wide, or targeted, screen.
     75Analyze CRISPR genome-wide, or targeted, screen.  As input, MAGeCK requires (raw) counts which are normalized.  If there are no replicates, MAGeCK will estimate the mean/variance from all the samples, i.e. both conditions.
    7676
    7777  * [[https://genomebiology.biomedcentral.com/articles/10.1186/s13059-014-0554-4 | Publication]]
    … …  
    9292# For paired samples:
    9393mageck test -k count_matrix.txt -t top1,top2 -c bot1,bot2 -n mageck_out.txt --paired
     94# note: --paired option is available in version 0.5.9+
    9495
    9596}}}