Changes between Version 17 and Version 18 of SOP/PooledCRISPR

Timestamp:

11/23/21 15:52:23 (3 years ago)

Author:

thiruvil

Comment:

--

SOP/PooledCRISPR

@@ -73 +73 @@
 == Method 2: MAGeCK ==
 
-Analyze CRISPR genome-wide, or targeted, screen.  As input, MAGeCK requires (raw) counts which are normalized.  If there are no replicates, MAGeCK will estimate the mean/variance from all the samples, i.e. both conditions.
+Analyze CRISPR genome-wide, or targeted, screen.  As input, MAGeCK requires (raw) counts.  If there are no replicates, MAGeCK will estimate the mean/variance from all the samples, i.e. both conditions.
 
   * [[https://genomebiology.biomedcentral.com/articles/10.1186/s13059-014-0554-4 | Publication]]
@@ -100 +100 @@
 
 
-{{{
-|sgRNA|gene|bot1|bot2|top1|top2|
-|sgACTL7A_2|ACTL7A|32|14|10|26|
-|sgACTL7A_3|ACTL7A|44|40|82|118|s
-|gACTL7A_4|ACTL7A|64|61|418|313|
-|sgACTL7A_5|ACTL7A|9|0|17|74|
-|sgACTL7A_6|ACTL7A|42|5|47|166|
-|sgACTL7A_7|ACTL7A|14|32|23|60|
-}}}
+||sgRNA||gene||bot1||bot2||top1||top2||
+||sgACTL7A_2||ACTL7A||32||14||10||26||
+||sgACTL7A_3||ACTL7A||44||40||82||118||
+||sgACTL7A_4||ACTL7A||64||61||418||313||
+||sgACTL7A_5||ACTL7A||9||0||17||74||
+||sgACTL7A_6||ACTL7A||42||5||47||166||
+||sgACTL7A_7||ACTL7A||14||32||23||60||