Changes between Version 1 and Version 2 of SOP/PooledCRISPR


Ignore:
Timestamp:
02/27/19 11:12:36 (6 years ago)
Author:
gbell
Comment:

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  • SOP/PooledCRISPR

    v1 v2  
    3434For each gene, the sgRNA with the lowest overall CRISPR score can be defined as the “best” sgRNA for that gene.
    3535
    36 Other details from the Wang et al. methods:
     36'''Other details from the Wang et al. methods:'''
    3737
    3838To identify genes essential for optimal proliferation under standard media conditions, the log2 fold change distribution for all sgRNAs targeting a given gene was compared with the entire distribution using a Kolmogorov-Smirnov test using the ks_2samp function from the scipy.stats Python library. The resulting p-values were corrected using the Benjamini-Hochberg procedure.