Changes between Version 7 and Version 8 of SOP/PooledCRISPR


Ignore:
Timestamp:
03/21/19 15:59:17 (6 years ago)
Author:
byuan
Comment:

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  • SOP/PooledCRISPR

    v7 v8  
    5252'''Implementation 2: using bowtie alignment'''
    5353* Make bowtie index with sgRNA sequences, and ran bowtie to align reads to indexed sgRNAs.
    54 * Sample commands with 0 mismatch in the alignment
     54
     55* Sample commands with 0 mismatch in the alignment:
    5556* bowtie-build guide.fa guide
    5657* bowtie  -3 20 -n 0  -l 20 -p 4  -S guide foo.fq.gz foo.sam