Changes between Version 1 and Version 2 of SOP/scRNA-seq/diffusionMaps
- Timestamp:
- 10/05/20 14:21:33 (4 years ago)
Legend:
- Unmodified
- Added
- Removed
- Modified
-
SOP/scRNA-seq/diffusionMaps
v1 v2 1 1 2 == = Making diffusion maps with destiny ===2 == Making diffusion maps with destiny == 3 3 4 4 Seurat v3 doesn't have a function to make diffusion maps anymore. The alternatives are to 1) run the analysis in python using scanpy or 2) make a single cell object, i.e. from the Seurat object, and run the "DiffusionMap" function from the destiny package. Example commands for the second option are shown below.