Changes between Version 48 and Version 49 of SOP/scRNA-seq


Ignore:
Timestamp:
10/28/21 09:54:11 (3 years ago)
Author:
ibarrasa
Comment:

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  • SOP/scRNA-seq

    v48 v49  
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     322Use the integrated assay to run umap visualization and jointly define cell types.  Use the RNA assay to identify canonical cell type marker genes and to show gene expression using FeaturePlot. Please check this vignette for more detail. [https://satijalab.org/seurat/archive/v3.0/immune_alignment.html]
    322323    * Using Harmony from within Seurat (please see I. Korsunsky ''et al.'' “Fast, sensitive and accurate integration of single-cell data with Harmony”, ''Nature Methods'' '''16''', 1289-1296 (2019), the details of the Harmony algorithm linked below and the documentation for the RunHarmony function):
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