Changes between Version 9 and Version 10 of SOPs/CUT&Tag


Ignore:
Timestamp:
04/24/24 18:04:14 (9 months ago)
Author:
ibarrasa
Comment:

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  • SOPs/CUT&Tag

    v9 v10  
    1919 mkdir /nfs/BaRC_training/CUTTAG/yourUserName
    2020 cd /nfs/BaRC_training/CUTTAG/yourUserName
    21  sbatch --partition=20 --job-name=NextF_CT --output=NextF_CT_1sample-%j.out  --mem=150gb   --nodes=1 --ntasks=1 --cpus-per-task=20 --wrap  "/nfs/BaRC_Public/apps/nextflow/nextflow run nf-core/cutandrun   -profile singularity  --input /nfs/BaRC_training/CUTTAG/nextFlow/samplesheet.csv  --normalization_mode RPGC --igg_scale_factor 1 --peakcaller 'MACS2'    --multiqc_title 'multiQCReport' --skip_removeduplicates true  --skiq_preseq false   --skip_dt_qc false --skip_multiqc false --skip_reporting false --dump_scale_factors true --email 'userName@wi.mit.edu' --genome  GRCh38 --extend_fragments false --macs2_qvalue 0.01 --minimum_alignment_q_score 0 --outdir  ./OutNextF_keepAllReads_RPGC_q0  "
     21 sbatch --partition=20 --job-name=NextF_CT --output=NextF_CT_1sample-%j.out  --mem=150gb   --nodes=1 --ntasks=1 --cpus-per-task=20 --wrap  " ../bin/nextflow run nf-core/cutandrun   -profile singularity  --input /nfs/BaRC_training/CUTTAG/nextFlow/samplesheet.csv  --normalisation_mode RPGC --igg_scale_factor 1 --peakcaller 'MACS2'    --multiqc_title 'multiQCReport' --skip_removeduplicates true  --skip_preseq false   --skip_dt_qc false --skip_multiqc false --skip_reporting false --dump_scale_factors true --email 'userName@wi.mit.edu' --genome  GRCh38 --extend_fragments false --macs2_qvalue 0.1 --minimum_alignment_q_score 0 --outdir  ./OutNextF_keepAllReads_RPGC_q0  "
    2222}}}
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