Changes between Version 4 and Version 5 of SOPs/CUT&Tag


Ignore:
Timestamp:
04/02/24 14:43:36 (10 months ago)
Author:
twhitfie
Comment:

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  • SOPs/CUT&Tag

    v4 v5  
    1010
    1111  * We recommend not removing duplicates from any of the samples.
    12   * Spike-in calibration using the number of fragments mapped to the E. coli genome, as described in the [https://yezhengstat.github.io/CUTTag_tutorial/ analysis protocol] published by the Henikoff lab, is be useful for visualization of the CUTTag profile on a genome browser.
     12  * Spike-in calibration using the number of fragments mapped to the E. coli genome, as described in the [https://yezhengstat.github.io/CUTTag_tutorial/ analysis protocol] published by the Henikoff lab, is useful for visualization of the CUT&Tag profile with a genome browser.
    1313  * Spike-in normalized bedgraph files are not an appropriate input for MACS2, since MACS2 will renormalize to the library size.
    1414