Changes between Version 8 and Version 9 of SOPs/CUT&Tag
- Timestamp:
- 04/23/24 14:55:39 (9 months ago)
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SOPs/CUT&Tag
v8 v9 19 19 mkdir /nfs/BaRC_training/CUTTAG/yourUserName 20 20 cd /nfs/BaRC_training/CUTTAG/yourUserName 21 sbatch --partition=20 --job-name=NextF_CT --output=NextF_CT_1sample-%j.out --mem=150gb --nodes=1 --ntasks=1 --cpus-per-task=20 --wrap " 21 sbatch --partition=20 --job-name=NextF_CT --output=NextF_CT_1sample-%j.out --mem=150gb --nodes=1 --ntasks=1 --cpus-per-task=20 --wrap "/nfs/BaRC_Public/apps/nextflow/nextflow run nf-core/cutandrun -profile singularity --input /nfs/BaRC_training/CUTTAG/nextFlow/samplesheet.csv --normalization_mode RPGC --igg_scale_factor 1 --peakcaller 'MACS2' --multiqc_title 'multiQCReport' --skip_removeduplicates true --skiq_preseq false --skip_dt_qc false --skip_multiqc false --skip_reporting false --dump_scale_factors true --email 'userName@wi.mit.edu' --genome GRCh38 --extend_fragments false --macs2_qvalue 0.01 --minimum_alignment_q_score 0 --outdir ./OutNextF_keepAllReads_RPGC_q0 " 22 22 }}} 23 23