Changes between Version 31 and Version 32 of SOPs/InProgress


Ignore:
Timestamp:
12/11/15 13:18:00 (9 years ago)
Author:
gbell
Comment:

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  • SOPs/InProgress

    v31 v32  
    1 == How long should your reads be?  Should they be single or paired-end? ==
     1== How long should the reads be?  Should they be single or paired-end? ==
    22
    33 * What is the goal of your experiment?
    … …  
    88
    99
    10 == If you are able to sequence more than one lane, how should the samples be divided? ==
     10== If you are able to sequence more than one lane, how should the samples be partitioned? ==
    1111
    12    * The magnitude of a lane effect is typically small but probably non-zero.
     12   * The magnitude of a lane effect is typically small but typically non-zero.
    1313   * To balance any lane effect, sequence all of your samples on each of your lanes.
    1414   * Another benefit of barcoding and mixing all samples together is that the samples can be re-sequenced in other lanes in the future (from the same library preparation) without unbalancing the experimental design.