Changes between Version 32 and Version 33 of SOPs/InProgress


Ignore:
Timestamp:
12/11/15 13:21:13 (8 years ago)
Author:
gbell
Comment:

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  • SOPs/InProgress

    v32 v33  
    66       * novel gene discovery: longer reads are much better at identifying novel splice junctions
    77   * For variant discovery, coverage is key, whether it's fewer long reads or more shorter reads (as long as the reads are long enough to map uniquely)
    8 
     8 * How much read length is used for primers, adapters, barcodes, etc.?  Of course make sure that enough actual experimental DNA is left for effective mapping.
    99
    1010== If you are able to sequence more than one lane, how should the samples be partitioned? ==
    … …  
    1313   * To balance any lane effect, sequence all of your samples on each of your lanes.
    1414   * Another benefit of barcoding and mixing all samples together is that the samples can be re-sequenced in other lanes in the future (from the same library preparation) without unbalancing the experimental design.
     15
     16== How many reads are needed for each sample? ==
    1517
    1618== Calculating number of DNA or RNA reads needed to obtain the desired coverage ==