Changes between Version 1 and Version 2 of SOPs/InProgressChipSeq
- Timestamp:
- 05/14/14 11:45:11 (11 years ago)
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SOPs/InProgressChipSeq
v1 v2 13 13 14 14 * See the [[http://barcwiki.wi.mit.edu/wiki/SOPs/mapping|mapping SOP]] for more details. 15 16 === Step 2: Call peaks (bound regions) === 15 === Step 2: Calculate strand cross correlation === 16 {{{ 17 Rscript /nfs/BaRC_Public/phantompeakqualtools/run_spp.R -c=TreatmentIP.bam -savp -out=TreatmentIP.run_spp.out 18 }}} 19 20 21 === Step 3: Call peaks (bound regions) === 17 22 Some of the parameters to consider when comparing programs are: 18 23 * Adjustment of sequence tags to better represent the original DNA fragment (by shifting tags in the 3′ direction or by extending tags to the estimated length of the original fragments)