Changes between Version 4 and Version 5 of SOPs/RRBS


Ignore:
Timestamp:
05/19/22 17:15:32 (3 years ago)
Author:
twhitfie
Comment:

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  • SOPs/RRBS

    v4 v5  
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    18   * **Quantification of methylation calls**
     18  * **Alignment and methylation calls**
    1919    * Bismark can (since version 0.6.beta1) use [https://www.nature.com/articles/nmeth.1923 bowtie2] to map short reads to reference genome(s).
    2020    * In the command below, bismark will expect to find C-to-T and a G-to-A versions of the reference genome.  From the following the steps above, these will be located within /nfs/genomes/myOrganism/bowtie/ (for Institute-wide reference genomes) or /path/to/custom/genome/bowtie for other reference genomes.