Changes between Version 7 and Version 8 of SOPs/RRBS
- Timestamp:
- 04/22/24 21:24:41 (9 months ago)
Legend:
- Unmodified
- Added
- Removed
- Modified
-
SOPs/RRBS
v7 v8 3 3 === Background === 4 4 5 *Reduced-representation bisulfite sequencing (RRBS) is an experimental [https://academic.oup.com/nar/article/33/18/5868/2401288 protocol] to measure and compare genomic methylation patterns. These experiments use one or multiple restriction enzymes on genomic DNA to produce sequence specific fragments that are subsequently treated with bisulfite and sequenced. The workflow below illustrates how to use [https://academic.oup.com/bioinformatics/article/27/11/1571/216956 bismark] to analyze RRBS data on the resources at the Whitehead Institute.5 Reduced-representation bisulfite sequencing (RRBS) is an experimental [https://academic.oup.com/nar/article/33/18/5868/2401288 protocol] to measure and compare genomic methylation patterns. These experiments use one or multiple restriction enzymes on genomic DNA to produce sequence specific fragments that are subsequently treated with bisulfite and sequenced. The workflow below illustrates how to use [https://academic.oup.com/bioinformatics/article/27/11/1571/216956 bismark] to analyze RRBS data on the resources at the Whitehead Institute. 6 6 7 7 === Step by step analysis ===