Changes between Version 103 and Version 104 of SOPs/atac_Seq


Ignore:
Timestamp:
04/07/23 12:21:22 (21 months ago)
Author:
byuan
Comment:

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  • SOPs/atac_Seq

    v103 v104  
    105105MACS v2 is applicable for ATAC-Seq using the appropriate options/parameters.
    106106
    107   * If you have human (hg38, hg19) and mouse (mm10, mm9) samples with biological replicates, you run [[https://github.com/ENCODE-DCC/atac-seq-pipeline|ENCODE ATAC-seq Pipeline]]. The pipeline takes fastq files, cleans and maps the reads, filters aligned reads and does peak calls. Here is the [[https://www.encodeproject.org/pipelines/ENCPL787FUN/|schema of the workflow]].  In addition, it does quality controls. Here is a [[http://barc.wi.mit.edu/education/hot_topics/ChIPseq_ATACseq_2021/qc.html | sample QC report]]. The steps below shows you how to run it on our Whitehead server.
     107  * If you have human (hg38, hg19) and mouse (mm10, mm9) samples with biological replicates, you run [[https://github.com/ENCODE-DCC/atac-seq-pipeline|ENCODE ATAC-seq Pipeline]]. The pipeline takes fastq files, cleans and maps the reads, filters aligned reads and does peak calls. Here is the [[https://www.encodeproject.org/pipelines/ENCPL787FUN/|schema of the workflow]].  In addition, it does quality controls. Here is a [[http://barc.wi.mit.edu/education/hot_topics/ChIPseq_ATACseq_2021/qc.html | sample QC report]]. The steps below shows you how to run it on our Whitehead server. Note: It only works on python2.
    108108      * content in input sample.json:
    109109{{{