Changes between Version 57 and Version 58 of SOPs/atac_Seq


Ignore:
Timestamp:
06/11/21 14:39:06 (4 years ago)
Author:
thiruvil
Comment:

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  • SOPs/atac_Seq

    v57 v58  
    186186}}}
    187187
    188 ==== Other Tools ====
    189 
    190 [[https://github.com/LiuLabUB/HMMRATAC | HMMRATAC]] is another piece of software for ATAC-seq peak-calling.
     188==== Other Tools or Options ====
     189
     190 *  [[https://github.com/LiuLabUB/HMMRATAC | HMMRATAC]]
     191 *  [[https://github.com/jsh58/Genrich | Genrich]]
     192 * Run MACS2 with BAMPE option for PE reads, i.e. -f BAMPE, this asks MACS pileup and infer the real fragment size from the alignment.  Note: this option can be used to find accessible regions but not suitable for finding exact cut sites.  Also, comparing this option with converting to BED format (above), the BAMPE peaks calls are more conservative and may miss real peaks.
     193
     194
    191195
    192196=== [=#Asessing Assessing Peak Calls ] ===