==== ChIP-Seq analysis packages ==== Most of the packages are from [[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Search&db=pubmed&term=19844228|Pepke et al., 2009]] (Table 1) [[chipSeqBakeOff| ChIP-Seq Bake-Off results]] To test: * CisGenome * ERANGE * [[http://liulab.dfci.harvard.edu/MACS/|MACS]] ([[http://liulab.dfci.harvard.edu/MACS/00README.html|README]]) ([[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Search&db=pubmed&term=18798982|Zhang et al., 2008]]) * PeakSeq (did not tried because it can only use input from ELAND) * SeqMonk * [[http://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/|SiSSRs]](Inma tried this instead of PeakSeq)([[http://nar.oxfordjournals.org/cgi/content/full/36/16/5221|Reference]]) ([[http://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/SISSRs-Manual.pdf|Manual]]) Others: * FindPeaks * F-Seq * GLITR * QuEST * SICER * spp * USeq * Garrett's SOLEXA_ENRICHED_REGIONS.py code Sample data for testing is from [[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Search&db=pubmed&term=18692474|Marson et al, 2008]], specifically [[http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE11724|GSE11724]] from NCBI GEO, including these samples (mapped to the mm9 (July 2007) genome), with Bowtie output in the files **/nfs/BaRC/ChIP_Seq_analysis/GSE11724/+.k3.best.map** and mapped as shown in **map_with_bowtie.sh** and also described in **Data_sources.txt** What assembly are WIG files from the publication? * GSM307140 (Nanog_mES_rep1) * GSM307141 (Nanog_mES_rep2) * GSM307147 (HEK4me3_mES_rep1) * GSM307148 (HEK4me3_mES_rep2) * GSM307149 (HEK4me3_mES_rep3) * GSM307155 (WCE_mES_rep1) * GSM307154 (WCE_mES_rep2) Outputs to share (all using July 2007 (mm9) mouse assembly): * Genome-wide BED or WIG file * BED file of peaks (preferably with some sort of scoring metric(s)) * Visualized output in IGV * Shell script of all executed commands (for reproducability) * Comments about the application and description of the method, including things such as these [[Questions about ChIP-Seq methods]]