Changes between Version 39 and Version 40 of SOPs/chip_seq_peaks
- Timestamp:
- 04/27/17 08:10:23 (8 years ago)
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SOPs/chip_seq_peaks
v39 v40 16 16 === Step 1: Map reads === 17 17 18 * Use [[http://bowtie-bio.sourceforge.net/tutorial.shtml|Bowtie]], [[http://bowtie-bio.sourceforge.net/bowtie2/index.shtml|bowtie2]], or another unspliced mapping tool. We recommend using Bowtie2 (default parameters), filtering to remove multi-mapped reads (eg. a mapping quality of 2 or higher) is not needed as Bowtie2 randomly chooses a best hit when multiple good hits exist (see [[http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#reporting | Bowtie2 Manual under Reporting ]]).18 * Use [[http://bowtie-bio.sourceforge.net/tutorial.shtml|Bowtie]], [[http://bowtie-bio.sourceforge.net/bowtie2/index.shtml|bowtie2]], or another unspliced mapping tool. We recommend using Bowtie2 (default parameters), filtering to remove multi-mapped reads (eg. a mapping quality of 2 or higher) is not needed as Bowtie2 randomly chooses a best hit when multiple good hits exist (see [[http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#reporting | Bowtie2 manual [under Reporting] ]). 19 19 20 20 * See the [[http://barcwiki.wi.mit.edu/wiki/SOPs/mapping|mapping SOP]] for more details. … … 112 112 113 113 ==== SISSRs ==== 114 [http://sissrs.rajajothi.com/ SiSSRs] ([https://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/SISSRs-Manual.pdf Manual])114 [http://sissrs.rajajothi.com/ SiSSRs] ([https://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/SISSRs-Manual.pdf manual]) 115 115 [[chipSeqBakeOff|ChIP-Seq bake off]] 116 116 … … 144 144 * -m is the percentage of mappable bps. Default is .8 for Eland in human. 145 145 146 For more detailed description of parameters see [[chipSeqBakeOff|ChIP-Seq bake off]]146 For more detailed description of parameters see our [chipSeqBakeOff ChIP-Seq bake off]. 147 147 148 148