Changes between Version 39 and Version 40 of SOPs/chip_seq_peaks


Ignore:
Timestamp:
04/27/17 08:10:23 (8 years ago)
Author:
gbell
Comment:

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  • SOPs/chip_seq_peaks

    v39 v40  
    1616=== Step 1: Map reads ===
    1717
    18  * Use [[http://bowtie-bio.sourceforge.net/tutorial.shtml|Bowtie]], [[http://bowtie-bio.sourceforge.net/bowtie2/index.shtml|bowtie2]], or another unspliced mapping tool. We recommend using Bowtie2 (default parameters), filtering to remove multi-mapped reads (eg. a mapping quality of 2 or higher) is not needed as Bowtie2 randomly chooses a best hit when multiple good hits exist (see [[http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#reporting | Bowtie2 Manual under Reporting ]]).
     18 * Use [[http://bowtie-bio.sourceforge.net/tutorial.shtml|Bowtie]], [[http://bowtie-bio.sourceforge.net/bowtie2/index.shtml|bowtie2]], or another unspliced mapping tool. We recommend using Bowtie2 (default parameters), filtering to remove multi-mapped reads (eg. a mapping quality of 2 or higher) is not needed as Bowtie2 randomly chooses a best hit when multiple good hits exist (see [[http://bowtie-bio.sourceforge.net/bowtie2/manual.shtml#reporting | Bowtie2 manual [under Reporting] ]).
    1919
    2020 * See the [[http://barcwiki.wi.mit.edu/wiki/SOPs/mapping|mapping SOP]] for more details.
     
    112112
    113113==== SISSRs ====
    114 [http://sissrs.rajajothi.com/ SiSSRs] ([https://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/SISSRs-Manual.pdf Manual])
     114[http://sissrs.rajajothi.com/ SiSSRs] ([https://dir.nhlbi.nih.gov/papers/lmi/epigenomes/sissrs/SISSRs-Manual.pdf manual])
    115115[[chipSeqBakeOff|ChIP-Seq bake off]]
    116116
     
    144144  * -m is the percentage of mappable bps. Default is .8 for Eland in human.
    145145
    146 For more detailed description of parameters see [[chipSeqBakeOff|ChIP-Seq bake off]]
     146For more detailed description of parameters see our [chipSeqBakeOff ChIP-Seq bake off].
    147147
    148148