Changes between Version 58 and Version 59 of SOPs/chip_seq_peaks
- Timestamp:
- 07/09/21 08:43:24 (4 years ago)
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SOPs/chip_seq_peaks
v58 v59 84 84 === [=#repro Step 4: Identify reproducible peaks with the IDR method [for peak-calling experiments with replication]] === 85 85 86 You can identify reproducible peaks with idrfunction, based on the method of [[https://projecteuclid.org/journals/annals-of-applied-statistics/volume-5/issue-3/Measuring-reproducibility-of-high-throughput-experiments/10.1214/11-AOAS466.full | Qunhua Li et al]] and using [[https://github.com/nboley/idr | code from Nathan Boley]].86 You can identify reproducible peaks with the 'idr' function, based on the method of [[https://projecteuclid.org/journals/annals-of-applied-statistics/volume-5/issue-3/Measuring-reproducibility-of-high-throughput-experiments/10.1214/11-AOAS466.full | Qunhua Li et al]] and using [[https://github.com/nboley/idr | code from Nathan Boley]]. 87 87 88 88 Start by running macs2 (macs version 2, not version 1) to call ChIP-seq peaks (see step 3)