| 213 | | Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an alternative method to ChIP-Seq: it improves on lower input, i.e. number of cells needed, and reducing antibody cross-reactivity. On average, sequencing depths of 3 to 5 million reads per sample should be sufficient, though depths of more than 15 million reads will increase duplication rates [[https://www.cellsignal.com/learn-and-support/frequently-asked-questions/cut-and-run-faqs#a26 | Cell Signal FAQ]]. Similar to ChIP-Seq, higher coverage is needed for histone marks compared to TF. |
| | 213 | Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an alternative method to ChIP-Seq: it improves on lower input, i.e. number of cells needed, and reduces antibody cross-reactivity. On average, sequencing depths of 3 to 5 million reads per sample should be sufficient, though depths of more than 15 million reads will increase duplication rates. For other details, see the [[https://www.cellsignal.com/learn-and-support/frequently-asked-questions/cut-and-run-faqs | Cell Signaling Technology FAQ]]. Similar to ChIP-Seq, higher coverage is needed for histone marks compared to transcription factor binding sites. |
