Changes between Version 77 and Version 78 of SOPs/chip_seq_peaks
- Timestamp:
- 11/09/21 10:24:10 (3 years ago)
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SOPs/chip_seq_peaks
v77 v78 236 236 * --nomodel and --extsize can be used after strand cross-correlation [see above] 237 237 238 Called peaks should be filtered based on FDR, e.g. q < 0.1, and other metrics, e.g. fold enrichment. If CUT&RUN samples had low coverage, e.g. less than ~3 million reads, [[https://github.com/FredHutch/SEACR|SEACR]] may be an alternative to MACS to call peaks.239 240 241 238 Called peaks should be filtered based on FDR, e.g. -log,,10,,(FDR) > 10, and other metrics, e.g. fold enrichment. If CUT&RUN samples had low coverage, e.g. less than ~3 million reads, [[https://github.com/FredHutch/SEACR|SEACR]] may be an alternative to MACS to call peaks. 239 240 241