Changes between Version 28 and Version 29 of SOPs/mapping

Timestamp:

05/31/16 15:19:57 (9 years ago)

Author:

gbell

Comment:

--

SOPs/mapping

@@ -145 +145 @@
 {{{
 # Single-end reads
-bsub tophat -o s_7_tophat_out --phred64-quals --segment-length 20 -I 200000 -G /nfs/genomes/mouse_gp_jul_07_no_random/gtf/Mus_musculus.NCBIM37.67_noNT.gtf --no-novel-juncs /nfs/genomes/mouse_gp_jul_07_no_random/bowtie/mm9 s_7.txt
+bsub tophat -o s_7_tophat_out --phred64-quals --library-type fr-firststrand --segment-length 20 -I 200000 -G /nfs/genomes/mouse_gp_jul_07_no_random/gtf/Mus_musculus.NCBIM37.67_noNT.gtf --no-novel-juncs /nfs/genomes/mouse_gp_jul_07_no_random/bowtie/mm9 s_7.txt
 # Paired-end reads
-bsub tophat -o s_7_tophat_out --phred64-quals --segment-length 20 -I 200000 -G /nfs/genomes/mouse_gp_jul_07_no_random/gtf/Mus_musculus.NCBIM37.67_noNT.gtf --no-novel-juncs /nfs/genomes/mouse_gp_jul_07_no_random/bowtie/mm9 s_7.1.txt s_7.2.txt
+bsub tophat -o s_7_tophat_out --phred64-quals --library-type fr-firststrand --segment-length 20 -I 200000 -G /nfs/genomes/mouse_gp_jul_07_no_random/gtf/Mus_musculus.NCBIM37.67_noNT.gtf --no-novel-juncs /nfs/genomes/mouse_gp_jul_07_no_random/bowtie/mm9 s_7.1.txt s_7.2.txt
 }}}
 
@@ -156 +156 @@
   * '''-G <GTF file>''' Supply bowtie with a GTF file of transcript models.  This can help bowtie identify functions that may otherwise be missed.
   * '''--no-novel-juncs ''' Only look for spliced reads across junctions in the supplied GTF file.  Not used if looking for novel isoforms.
+  * '''--library type ''' Take advantage of strandedness of library for mapping (especially across splice junctions); can be fr-unstranded, fr-firststrand, or fr-secondstrand
 
 Choices for fastq encoding (which is listed as "Encoding" in the top "Basic Statistics" table of the FastQC output file).  See the [http://en.wikipedia.org/wiki/FASTQ_format FASTQ format page] for more details.