Changes between Version 50 and Version 51 of SOPs/mapping
- Timestamp:
- 06/09/17 09:44:08 (8 years ago)
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SOPs/mapping
v50 v51 228 228 * '''--outSAMtype <BAM sortingMode> ''' Specifies the type of BAM file to create. Options: 'BAM Unsorted', 'BAM SortedByCoordinate', 'BAM Unsorted SortedByCoordinate' (to create both unsorted and sorted BAMs) 229 229 230 To improve identification of novel splice junctions, run STAR for a second pass, using junctions found from all samples during the first-pass mapping. 230 To improve identification of novel splice junctions, run STAR for a second pass, using junctions found from all samples during the first-pass mapping. This is especially useful if no GTF file (with annotated junctions) was available for the first-pass mapping. 231 231 232 232 Sample second-pass command: