Changes between Version 69 and Version 70 of SOPs/mapping
- Timestamp:
- 09/21/20 16:00:08 (5 years ago)
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SOPs/mapping
v69 v70 2 2 == Mapping short reads == 3 3 4 5 6 === Regular mappers === 7 === [#splice_mappers Go to splice-aware mappers] === 8 9 Some choices for regular short-read mappers are 10 * [#bwa BWA] 11 * [#bowtie2 bowtie2] 12 * [#bowtie bowtie] 13 4 '''Contents:''' 5 * [#regular Regular mappers] 6 * [#bwa BWA] 7 * [#bowtie2 bowtie2] 8 * [#bowtie bowtie] 9 * [#splice_mappers Splice-aware mappers] 10 * [#STAR STAR] 11 * [#tophat2 Tophat 2] 12 * [#tophat Tophat] 13 * ''[#others others]'' 14 15 === [=#regular Regular mappers] === 14 16 15 17 These mapping tools are useful for reads of DNA origin that should map to a continuous stretch of genomic DNA. Some of these tools can tolerate short indels but they're not designed for reads that span a splice junction … … 110 112 These mappers permit the beginning and end of a read to map to (originate from) different places in the genome, which is common for spliced RNA. 111 113 112 Some choices for regular short-read mappers are 113 * [#STAR STAR] 114 * [#tophat2 tophat2] 115 * [#tophat tophat] 114 Some choices for regular short-read mappers are [#STAR STAR], [#tophat2 Tophat 2], and [#tophat Tophat]. 116 115 117 116 === [=#STAR STAR] === … … 258 257 * '''--solexa1.3-quals''' or '''--phred64-quals''' (for input quality scores from Illumina versions 1.3-1.7) 259 258 260 == Others==259 == [=#others Others] == 261 260 262 261 * [[https://daehwankimlab.github.io/hisat2/ | HISAT2]]: successor of TopHat2/HISAT