Changes between Version 20 and Version 21 of SOPs/miningSAMBAM


Ignore:
Timestamp:
11/17/14 10:43:37 (10 years ago)
Author:
thiruvil
Comment:

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  • SOPs/miningSAMBAM

    v20 v21  
    9797=== QC to get a (visual) summary of mapping statistics.  For eg. coverage/distribution of mapped reads across the genome or transcriptome. ===
    9898
    99 ==== RSeQC: RNA-Seq quality control package for getting mapping statistics (eg. unique/multi-mapped reads) ====
     99==== [http://rseqc.sourceforge.net/ | RSeQC]: RNA-Seq quality control package for getting mapping statistics (eg. unique/multi-mapped reads) ====
    100100{{{
    101101bam_stat.py -i myFile.bam
    102102}}}
    103103
    104 ==== Picard: CollectRnaSeqMetrics.jar to find coverage across gene body for 5' or 3' bias ====
     104==== [http://broadinstitute.github.io/picard/ | Picard]: CollectRnaSeqMetrics.jar to find coverage across gene body for 5' or 3' bias ====
    105105{{{
    106106java -jar /usr/local/share/picard-tools/CollectRnaSeqMetrics.jar INPUT=accepted_hits.bam REF_FLAT=refFlat.txt STRAND_SPECIFICITY=NONE OUTPUT=Out_RnaSeqMetrics.txt REFERENCE_SEQUENCE=hg19.fa CHART_OUTPUT=Out_RnaSeqMetrics.pdf
    107107}}}
    108108
    109 ==== QualiMap: can be used on DNA or RNA-Seq to get summary of mapping and coverage/distribution ====
     109==== [http://qualimap.bioinfo.cipf.es/ | QualiMap]: can be used on DNA or RNA-Seq to get summary of mapping and coverage/distribution ====
    110110{{{
    111111# Graphical interface: enter 'qualimap' on the command line