Changes between Version 39 and Version 40 of SOPs/miningSAMBAM
- Timestamp:
- 12/05/17 10:17:53 (7 years ago)
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SOPs/miningSAMBAM
v39 v40 181 181 bsub "samtools view -b -F 0x0040 accepted_hits.bam > accepted_hits_2ndPair.bam" 182 182 }}} 183 184 === Print consensus sequence of mapped reads === 185 {{{ 186 samtools mpileup -uf ref_genome.fa Mapped_reads.bam >| Mapped_reads.variants.bcf 187 bcftools call -O v -c Mapped_reads.variants.bcf | vcfutils.pl vcf2fq -d 2 >| Mapped_reads.consensus.fq 188 }}}