Changes between Version 11 and Version 12 of SOPs/qc_SRA
- Timestamp:
- 10/20/20 11:04:08 (4 years ago)
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SOPs/qc_SRA
v11 v12 77 77 If you don't specify an output directory, the SRR files will be downloaded to ~/ncbi/ncbi_public/sra (or your configured "Import Path" as described above). 78 78 79 To get this list of SRR IDs, go the [[https://www.ncbi.nlm.nih.gov/Traces/study/|SRA Run Selector]] and enter a project accession. Once on a [[https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP000002|project page]], go to the "Select" section and click on "Accession List" to get 'SRR_Acc_List.txt' (or if you want a subset of these, click on "Metadata" in the "Select" section to get a comma separated file, 'SraRunTable.txt' and create your own 'SRR_Acc_List.txt') 79 To get this list of SRR IDs, go the [[https://www.ncbi.nlm.nih.gov/Traces/study/|SRA Run Selector]] and enter a project accession. Once on a [[https://www.ncbi.nlm.nih.gov/Traces/study/?acc=SRP000002|project page]], go to the "Select" section and click on "Accession List" to get 'SRR_Acc_List.txt' (or if you want a subset of these, click on "Metadata" in the "Select" section to get a comma separated file, 'SraRunTable.txt' and create your own 'SRR_Acc_List.txt'). Or you can go to the [[https://trace.ncbi.nlm.nih.gov/Traces/sra/sra.cgi?view=search_seq_name|Downloading SRA data]] page, enter a list of SRX (experimental accession) IDs, and get a list of SRR (sample) accessions for 'SRR_Acc_List.txt'. 80 80 81 81 The 'prefetch' command will provide you with a set of SRA files which then need to be converted to fastq.gz. One way to do this on the set of SRA files is