=== NOISeq === * For experiments with or without replication. * Compares replicates within the same condition to estimate noise distribution of M (log-ratio ) and D (absolute value of the difference). A feature is considered to be differentially expressed if its corresponding M and D values are likely to be higher than noise values. * Input can be raw or normalized counts. * It has several normalization options: counts per million read, RPKM, Upper Quartile. It can also use pre-normalized data, for example, data normalized with DESeq. * Output statistic is probability of being differentially expressed. So, the higher and the closer to 1 the better. (It is NOT a p-value). * Sample code with replication: {{{ source("NOISeq.r") mydata <- readData(file = "All_Counts_no0_1pc_Header.txt", cond1 = c(2:3), cond2 = c(4:5), header = TRUE) myresults <- noiseq(mydata[[1]], mydata[[2]], repl = "bio", q = 0.9, nss = 0) write.table(cbind (myresults$Ms[myresults$deg],myresults$probab[myresults$deg] ), file="genesDE_FCUHRvbrain.txt", quote=F, sep="\t") #nss = 0 If the experiment didn’t include replicas the number of replicates to be simulated is provided by nss parameter #repl = "bio“ indicates that the experiment includes biological replicates #q = 0.9 indicates that the probability cut off for considering a gene differentially expressed is 0.9 }}}