Changes between Version 9 and Version 10 of SOPs/rna-seq-diff-expressions
- Timestamp:
- 08/23/13 10:21:49 (11 years ago)
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SOPs/rna-seq-diff-expressions
v9 v10 96 96 * Unless otherwise noted, these statistical analyses require raw counts, not RPKM values, as input. 97 97 * To prepare the data for analysis, make a counts matrix (as a tab-delimited text file) with genes as rows and samples as columns. 98 * Some statistical tests or packages can be applied to non-replicated data (but must be interpreted with caution):98 * Some statistical tests or packages can be applied to non-replicated data (but must be interpreted with a high degree of caution): 99 99 * [[http://cufflinks.cbcb.umd.edu/manual.html#cuffdiff|cuffDiff]] (part of the cufflinks package; uses BAM files as input) 100 100 * [[http://www.bioconductor.org/packages/release/bioc/html/DESeq.html|DESeq]] [wiki:SOPs/rna-seq-diff-expressions/DESeq | More detail about DESeq]