Changes between Version 10 and Version 11 of SOPs/rna-seq-diff-expressions
- Timestamp:
- 08/23/13 10:23:44 (11 years ago)
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SOPs/rna-seq-diff-expressions
v10 v11 99 99 * [[http://cufflinks.cbcb.umd.edu/manual.html#cuffdiff|cuffDiff]] (part of the cufflinks package; uses BAM files as input) 100 100 * [[http://www.bioconductor.org/packages/release/bioc/html/DESeq.html|DESeq]] [wiki:SOPs/rna-seq-diff-expressions/DESeq | More detail about DESeq] 101 * [[http://www.bioconductor.org/packages/release/bioc/html/DEGseq.html|DEGSeq]]102 101 * [[http://bioinfo.cipf.es/noiseq/doku.php?id=tutorial|NOISeq]] [wiki:SOPs/rna-seq-diff-expressions/NOISeq | More detail about NOISeq] 103 102 * Fisher's exact test (with R and/or with BaRC R script) [wiki:SOPs/rna-seq-diff-expressions/Fisher | More detail about Fisher's exact test] … … 105 104 * [[http://www.bioconductor.org/packages/release/bioc/html/DESeq.html|DESeq]] [wiki:SOPs/rna-seq-diff-expressions/DESeq | More detail about DESeq] 106 105 * [[http://www.bioconductor.org/packages/release/bioc/html/edgeR.html|edgeR]] [wiki:SOPs/rna-seq-diff-expressions/EdgeR | More detail about EdgeR] 107 * [[http://www.bioconductor.org/packages/release/bioc/html/baySeq.html|baySeq]]108 106 * [[http://bioinfo.cipf.es/noiseq/doku.php?id=tutorial|NOISeq]] [wiki:SOPs/rna-seq-diff-expressions/NOISeq | More detail about NOISeq] 109 107 * In our hands, all of these methods appear to work fine. Note, however, that the scale of the p-values from baySeq are much different from those of DESeq and edgeR. For example, a gene may have a baySeq p-value of 1e-15 but a DESeq p-value of 1e-300.