Changes between Version 30 and Version 31 of SOPs/rna-seq-diff-expressions


Ignore:
Timestamp:
06/01/16 10:46:28 (9 years ago)
Author:
gbell
Comment:

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  • SOPs/rna-seq-diff-expressions

    v30 v31  
    3434      * Counting can be also performed transcript level but this may be too much information.
    3535      * htseq-count can accept SAM or BAM (-f option), for example
    36          * ''htseq-count -f bam -m intersection-strict --stranded=reverse accepted_hits.sortedByName.bam gene_models.gtf >| gene_model.counts.txt''
     36         * ''htseq-count -f bam -m intersection-strict --stranded=reverse MySample.accepted_hits.sortedByName.bam gene_models.gtf >| MySample.counts.txt''
    3737         * Note that htseq-count assumes that your reads are strand-specific; default is --stranded=yes
    3838         * If your reads are not stranded, use "--stranded=no" (or half of your reads won't be counted).  If they're stranded and in the opposite direction from the transcript, use "--stranded=reverse".