Changes between Version 35 and Version 36 of SOPs/rna-seq-diff-expressions
- Timestamp:
- 04/27/17 08:20:51 (8 years ago)
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SOPs/rna-seq-diff-expressions
v35 v36 79 79 * As with microarray normalization, be aware of the assumptions of each method and choose the method(s) which are most valid with your experiment. 80 80 * Origin of recommendation for upper-quartile normalization: [[http://www.ncbi.nlm.nih.gov/pubmed/20167110|Bullard et al., 2010]] 81 * See [[http://jura.wi.mit.edu/bio/scripts/R/normalize_DGE_matrix.R|normalize_DGE_matrix.R]] for a command-line script for count normalization81 * Use DESeq, DESeq2, or see [[http://jura.wi.mit.edu/bio/scripts/R/normalize_DGE_matrix.R|normalize_DGE_matrix.R]] for a command-line script for count normalization 82 82 * Methods for above script: mean, median, quantile, percentile, none 83 83 * Outputs also includes before and after image files: boxplots, scatterpots, violin plots