Changes between Version 40 and Version 41 of SOPs/rna-seq-diff-expressions
- Timestamp:
- 05/17/17 10:47:36 (8 years ago)
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SOPs/rna-seq-diff-expressions
v40 v41 67 67 * **NOTE:** 68 68 * Both htseq-count and featureCounts ignore multi-mapped reads (ie. these will not get counted) by default. In featureCounts use -M option to count multi-mapped reads, if needed. 69 * Summary reported by both htseq-count (at the bottom of output file) and featureCounts is with respect to number of records (ie. lines) in the bam file, to summarize per read further parsing may be needed: extra information can be obtained from i) htseq-count use -o option and ii) featureCounts use -R option.69 * Summary stats reported on reads/features counted by both htseq-count (at the bottom of output file) and featureCounts is with respect to number of records (ie. lines) in the bam file, to summarize per read further parsing may be needed: extra information can be obtained from i) htseq-count use -o option and ii) featureCounts use -R option. 70 70 71 71