Changes between Version 59 and Version 60 of SOPs/rna-seq-diff-expressions


Ignore:
Timestamp:
10/16/18 10:11:37 (6 years ago)
Author:
gbell
Comment:

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  • SOPs/rna-seq-diff-expressions

    v59 v60  
    3030
    3131    * Is your sequencing library stranded or unstranded?  This information is needed to help these tools accurately count features.  Strandedness of some library prep methods:
    32       * "TruSeqStrandedPolyA" reads are stranded in the reverse direction (relative to the transcript orientation).
    33       * "SMARTerUltra-lowPOLYA-V4" reads are unstranded.
     32      * TruSeq Stranded mRNA Kits ("TruSeqStrandedPolyA") reads are reverse stranded (stranded in the reverse direction relative to the transcript orientation).
     33      * SMART-Seq v4 Ultra Low Input RNA Kit ("SMARTerUltra-lowPOLYA-V4") reads are unstranded.
     34      * KAPA RNA HyperPrep Kits ("KAPAHyperPrepmRNA") reads are reverse stranded.
    3435    * See [[SAMBAMqc]] (and/or look at mapped reads in a genome browser) to determine or verify strandedness
    3536