Changes between Version 3 and Version 4 of SOPs/submittingSequencingToGEO


Ignore:
Timestamp:
02/16/24 10:45:52 (11 months ago)
Author:
gbell
Comment:

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  • SOPs/submittingSequencingToGEO

    v3 v4  
    32321. Right before or after your publication is made public, change the GEO access from private to public.  [[br]] [[br]]
    3333
     34To upload your files to GEO, you can use a file transfer program with a graphical interface (as recommended by GEO), or you can use a command-line method on a Whitehead server.  Two options are ftp and ncftp.  In the simplest case, all of your files for submission will be in the same directory.  Start by going to that directory on the WI server and then confirm that the files are actually there.
    3435
     36{{{
     37cd /path/to/my/files
     38ls
     39}}}
     40
     41Make a connection to the NCBI server using the credentials that you got from NCBI and upload your files.
     42
     43
     44{{{
     45# Using FTP
     46ftp ftp-private.ncbi.nlm.nih.gov
     47# You'll be prompted for your username and password
     48name: geoftp
     49password: MYPASSWD  # each submission is different
     50# Now you should be connected to the GEO server.  Change to the directory you were given
     51cd uploads/jsmith_e123ABC
     52# Turn off iterative mode (so you don't get asked to confirm every file)
     53prompt
     54# Upload all of the files in the directory from where you connected to GEO
     55mput *
     56# Wait what can be a long time ...
     57# After everything is uploaded
     58quit
     59}}}
     60
     61{{{
     62# Using NCFTP
     63ncftp -u geoftp -p MYPASSWD ftp-private.ncbi.nlm.nih.gov
     64# Now you should be connected to the GEO server.  Change to the directory you were given
     65cd uploads/jsmith_e123ABC
     66# Upload all of the directories/files in the directory from where you connected to GEO
     67# Note that this (a recursive upload) is something that 'ftp' can't do
     68mput -r *
     69# Wait what can be a long time ...
     70# After everything is uploaded
     71quit
     72}}}
     73
     74While you command-line uploading is in progress, you can always open a FTP GUI (like FileZilla) to monitor and/or confirm progress.
     75
     76