Changes between Version 4 and Version 5 of SOPs/variant_calling_GATK


Ignore:
Timestamp:
01/16/14 15:55:20 (11 years ago)
Author:
gbell
Comment:

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  • SOPs/variant_calling_GATK

    v4 v5  
    2626  * bsub "bwa samse /path/to/genome/bwa/genome Reads_1.sai  Reads_1.fq > Reads_1.bwa.sam"
    2727\\
    28 '''Convert SAM to BAM, sort, and index''' with [[http://samtools.sourceforge.net/samtools.shtml|samtools]]
     28'''Convert SAM to BAM, sort, and index''' with BaRC's streamlined [[http://samtools.sourceforge.net/samtools.shtml|samtools]] commands
    2929  * bsub /nfs/BaRC_Public/BaRC_code/Perl/SAM_to_BAM_sort_index/SAM_to_BAM_sort_index.pl Reads_1.bwa.sam
    3030\\
    31 '''Mark duplicates''' (multiple identical reads mapped to the same location). \\
     31'''Mark duplicates''' (multiple identical reads mapped to the same location) \\
    3232Run Picard Tools' [[http://picard.sourceforge.net/command-line-overview.shtml#MarkDuplicates|MarkDuplicates]] on each sample \\
    3333May Need "VALIDATION_STRINGENCY=LENIENT" if you get  \\