Changes between Version 4 and Version 5 of SOPs/variant_calling_GATK
- Timestamp:
- 01/16/14 15:55:20 (11 years ago)
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SOPs/variant_calling_GATK
v4 v5 26 26 * bsub "bwa samse /path/to/genome/bwa/genome Reads_1.sai Reads_1.fq > Reads_1.bwa.sam" 27 27 \\ 28 '''Convert SAM to BAM, sort, and index''' with [[http://samtools.sourceforge.net/samtools.shtml|samtools]]28 '''Convert SAM to BAM, sort, and index''' with BaRC's streamlined [[http://samtools.sourceforge.net/samtools.shtml|samtools]] commands 29 29 * bsub /nfs/BaRC_Public/BaRC_code/Perl/SAM_to_BAM_sort_index/SAM_to_BAM_sort_index.pl Reads_1.bwa.sam 30 30 \\ 31 '''Mark duplicates''' (multiple identical reads mapped to the same location) .\\31 '''Mark duplicates''' (multiple identical reads mapped to the same location) \\ 32 32 Run Picard Tools' [[http://picard.sourceforge.net/command-line-overview.shtml#MarkDuplicates|MarkDuplicates]] on each sample \\ 33 33 May Need "VALIDATION_STRINGENCY=LENIENT" if you get \\