Download nSolver, follow nSOlver guide for Check QC, normalization and analysis.

For mRNA normalization, nSolver first checks on positive controls, and then normalizes housekeeping genes with geometric mean.

The reported ratio is fold change, with the higher value as numerator, sign as direction of the comparison.

You can also analyze directly with raw feature counts or normalized values downloaded from nSolver.

Note: See TracWiki for help on using the wiki.