Changes between Version 16 and Version 17 of SOPs/SAMBAMqc
- Timestamp:
- 02/03/17 15:30:55 (8 years ago)
Legend:
- Unmodified
- Added
- Removed
- Modified
-
SOPs/SAMBAMqc
v16 v17 139 139 # For gene counting with htseq-count, use --stranded=no; mapping with TopHat should have been performed with --library-type=fr-unstranded. 140 140 141 #sample output on stranded SE reads: 142 This is SingleEnd Data 143 Fraction of reads failed to determine: 0.0068 144 Fraction of reads explained by "++,--": 0.0068 145 Fraction of reads explained by "+-,-+": 0.9865 146 # For gene counting with htseq-count, use --stranded=reverse; mapping with TopHat should have been performed with --library-type=fr-firststrand. 147 148 141 149 For pair-end RNA-seq, there are two different ways to strand reads: 142 150 i) 1++,1--,2+-,2-+