Changes between Version 16 and Version 17 of SOPs/SAMBAMqc


Ignore:
Timestamp:
02/03/17 15:30:55 (8 years ago)
Author:
thiruvil
Comment:

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  • SOPs/SAMBAMqc

    v16 v17  
    139139# For gene counting with htseq-count, use --stranded=no; mapping with TopHat should have been performed with --library-type=fr-unstranded.
    140140
     141#sample output on stranded SE reads:
     142This is SingleEnd Data
     143Fraction of reads failed to determine: 0.0068
     144Fraction of reads explained by "++,--": 0.0068
     145Fraction of reads explained by "+-,-+": 0.9865
     146# For gene counting with htseq-count, use --stranded=reverse; mapping with TopHat should have been performed with --library-type=fr-firststrand.
     147
     148
    141149For pair-end RNA-seq, there are two different ways to strand reads:
    142150  i) 1++,1--,2+-,2-+