Changes between Version 20 and Version 21 of SOPs/SAMBAMqc
- Timestamp:
- 04/26/17 11:18:25 (8 years ago)
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SOPs/SAMBAMqc
v20 v21 17 17 If paired-end insert size or distance is unknown or need to be verified, it can be extracted from a BAM/SAM file after running Bowtie. 18 18 19 When mapping with bowtie (or another mapper), the insert size can often be included as an input parameter (example for bowtie: -X 500), which can help with mapping. See the [[http://barcwiki.wi.mit.edu/wiki/SOPs/mapping mapping SOP]] for mapping details.20 19 When mapping with bowtie (or another mapper), the insert size can often be included as an input parameter (example for bowtie: -X 500), which can help with mapping. 20 See our [http://barcwiki.wi.mit.edu/wiki/SOPs/mapping mapping SOP] for mapping details. 21 21 22 22 Method 1: Get insert sizes from BAM file … … 196 196 == Interpreting quality control issues == 197 197 198 See [ [https://sequencing.qcfail.com/ QCFAIL.com]] from the Babraham Institute198 See [https://sequencing.qcfail.com/ QCFAIL.com] from the Babraham Institute 199 199 200 200 \\