Changes between Version 12 and Version 13 of SOPs/qc_shortReads
- Timestamp:
- 01/16/14 11:33:45 (11 years ago)
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SOPs/qc_shortReads
v12 v13 6 6 7 7 \\ 8 9 == Quality scoring introduction == 10 11 * Quality scores are typically represented using a Phred scoring scheme, where read quality = −10 * log10(error) 12 * For example, 13 * Quality = 10 => error rate = 10% => base call has 90% confidence 14 * Quality = 20 => error rate = 1% => base call has 99% confidence 15 * Quality = 30 => error rate = 0.1% => base call has 99.9% confidence 16 * See [[http://en.wikipedia.org/wiki/Phred_quality_score|Phred quality score]] for more details. 8 17 9 18 == FastQC ==