Changes between Version 16 and Version 17 of SOPs/qc_shortReads
- Timestamp:
- 09/15/14 17:28:12 (10 years ago)
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SOPs/qc_shortReads
v16 v17 184 184 * much more conservative than fastx_clipper. 185 185 * [wiki:SOPs/cutadapt sample usage] 186 * a vs b options from [[http://journal.embnet.org/index.php/embnetjournal/article/view/200|EMBnet.journal]] 187 [[Image(cutadapt.2.jpeg,500px)]] 186 188 * sample command: 187 189 188 {{{ 189 bsub cutadapt -a GATCGGAAGAGCTCGTATGCCGTCTT –o Nanog_noAdapter.fastq Nanog.fastq 190 191 In the above command: 192 -a: Sequence of an adapter that was ligated to the 3' end. 193 -o: output file name 194 }}} 190 {{{ 191 bsub cutadapt -a GATCGGAAGAGCTCGTATGCCGTCTT –o Nanog_noAdapter.fastq Nanog.fastq 192 193 In the above command: 194 -a: Sequence of an adapter that was ligated to the 3' end. 195 -o: output file name 196 }}} 197 195 198 196 199 * See [[http://hannonlab.cshl.edu/fastx_toolkit/commandline.html#fastx_clipper_usage|fastx_clipper usage]] (or ''fastx_clipper -h'') for more arguments