Changes between Version 16 and Version 17 of SOPs/qc_shortReads


Ignore:
Timestamp:
09/15/14 17:28:12 (10 years ago)
Author:
thiruvil
Comment:

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  • SOPs/qc_shortReads

    v16 v17  
    184184    * much more conservative than fastx_clipper.
    185185    * [wiki:SOPs/cutadapt sample usage]
     186    * a vs b options from [[http://journal.embnet.org/index.php/embnetjournal/article/view/200|EMBnet.journal]]
     187       [[Image(cutadapt.2.jpeg,500px)]]
    186188    * sample command:
    187189
    188 {{{
    189 bsub cutadapt -a GATCGGAAGAGCTCGTATGCCGTCTT –o Nanog_noAdapter.fastq Nanog.fastq
    190 
    191 In the above command:
    192    -a: Sequence of an adapter that was ligated to the 3' end.
    193    -o:  output file name
    194 }}}
     190    {{{
     191    bsub cutadapt -a GATCGGAAGAGCTCGTATGCCGTCTT –o Nanog_noAdapter.fastq Nanog.fastq
     192
     193    In the above command:
     194       -a: Sequence of an adapter that was ligated to the 3' end.
     195       -o:  output file name
     196    }}}
     197
    195198
    196199  * See [[http://hannonlab.cshl.edu/fastx_toolkit/commandline.html#fastx_clipper_usage|fastx_clipper usage]] (or ''fastx_clipper -h'') for more arguments