Changes between Version 34 and Version 35 of SOPs/qc_shortReads


Ignore:
Timestamp:
04/26/17 10:54:44 (8 years ago)
Author:
gbell
Comment:

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  • SOPs/qc_shortReads

    v34 v35  
    1212== Naming ==
    1313
    14 FASTQ header/naming scheme as specified by, \\
     14FASTQ header/naming scheme as specified by \\
     15
    1516[[http://support.illumina.com/help/SequencingAnalysisWorkflow/Content/Vault/Informatics/Sequencing_Analysis/CASAVA/swSEQ_mCA_FASTQFiles.htm | Illumina (Casava 1.8.2):]]\\
    1617@<instrument>:<run number>:<flowcell ID>:<lane>:<tile>:<x-pos>:<y-pos> <read>:<is filtered>:<control number>:<index sequence>
     
    3031||<index sequence>||ACTG||Index sequence||
    3132\\
    32 
    3333Illumina (Casava 1.7)\\
    3434@<machine_id>:<lane>:<tile>:<x_coord>:<y_coord>#<index>/<read_#>
     
    6060[[Image(fastq_phread-base.png)]]
    6161
    62   from [[https://en.wikipedia.org/wiki/FASTQ_format | Wiki FASTQ Format]]
     62  from [[https://en.wikipedia.org/wiki/FASTQ_format | Wikipedia's FASTQ page]]
    6363
    6464== FastQC ==
    6565
    66 http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc
     66http://www.bioinformatics.babraham.ac.uk/projects/fastqc/
    6767  * quality control analysis with nice graphical output
    6868  * available for Linux, Windows, and MacOSX
     
    192192
    193193
    194   * [[http://code.google.com/p/cutadapt/|cutadapt]] is a good tool that is designed to find and remove adapters:
     194  * [[https://cutadapt.readthedocs.io/en/stable/|cutadapt]] is a good tool that is designed to find and remove adapters:
    195195    * more options than fastx_clipper, such as specifically trimming 5' or 3' adapters and specifying error rate (allowed mismatches)
    196196    * much more conservative than fastx_clipper.
     
    264264== Galaxy ==
    265265
    266 http://main.g2.bx.psu.edu/
     266https://usegalaxy.org/
    267267Many functions \\
    268268