Changes between Version 34 and Version 35 of SOPs/qc_shortReads
- Timestamp:
- 04/26/17 10:54:44 (8 years ago)
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SOPs/qc_shortReads
v34 v35 12 12 == Naming == 13 13 14 FASTQ header/naming scheme as specified by, \\ 14 FASTQ header/naming scheme as specified by \\ 15 15 16 [[http://support.illumina.com/help/SequencingAnalysisWorkflow/Content/Vault/Informatics/Sequencing_Analysis/CASAVA/swSEQ_mCA_FASTQFiles.htm | Illumina (Casava 1.8.2):]]\\ 16 17 @<instrument>:<run number>:<flowcell ID>:<lane>:<tile>:<x-pos>:<y-pos> <read>:<is filtered>:<control number>:<index sequence> … … 30 31 ||<index sequence>||ACTG||Index sequence|| 31 32 \\ 32 33 33 Illumina (Casava 1.7)\\ 34 34 @<machine_id>:<lane>:<tile>:<x_coord>:<y_coord>#<index>/<read_#> … … 60 60 [[Image(fastq_phread-base.png)]] 61 61 62 from [[https://en.wikipedia.org/wiki/FASTQ_format | Wiki FASTQ Format]]62 from [[https://en.wikipedia.org/wiki/FASTQ_format | Wikipedia's FASTQ page]] 63 63 64 64 == FastQC == 65 65 66 http://www.bioinformatics.b bsrc.ac.uk/projects/fastqc66 http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ 67 67 * quality control analysis with nice graphical output 68 68 * available for Linux, Windows, and MacOSX … … 192 192 193 193 194 * [[http ://code.google.com/p/cutadapt/|cutadapt]] is a good tool that is designed to find and remove adapters:194 * [[https://cutadapt.readthedocs.io/en/stable/|cutadapt]] is a good tool that is designed to find and remove adapters: 195 195 * more options than fastx_clipper, such as specifically trimming 5' or 3' adapters and specifying error rate (allowed mismatches) 196 196 * much more conservative than fastx_clipper. … … 264 264 == Galaxy == 265 265 266 http ://main.g2.bx.psu.edu/266 https://usegalaxy.org/ 267 267 Many functions \\ 268 268