Changes between Version 45 and Version 46 of SOPs/qc_shortReads


Ignore:
Timestamp:
09/17/18 16:38:10 (6 years ago)
Author:
thiruvil
Comment:

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  • SOPs/qc_shortReads

    v45 v46  
    202202  * What is the sequence of the linker (adapter) to be removed?
    203203    * Biologists generally know which linker (adapter) RNA is used for their sample(s).
    204     * For experiments using Illumina libraries, [[https://support.illumina.com/bulletins/2016/12/what-sequences-do-i-use-for-adapter-trimming.html|What sequences do I use for adapter trimming?]] may help you find the correct adapter sequence(s).
     204    * For experiments using Illumina libraries, [[https://support.illumina.com/bulletins/2016/12/what-sequences-do-i-use-for-adapter-trimming.html|What sequences do I use for adapter trimming?]] may help you find the correct adapter sequence(s).  The above adapters may be used with KAPA kits as well.
    205205    * Also or in addition, when you run quality control with shortRead or FASTQC, check out
    206206         * repetitive segments in the "over represented sequences" section.