Changes between Version 48 and Version 49 of SOPs/qc_shortReads
- Timestamp:
- 02/20/19 15:02:15 (6 years ago)
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SOPs/qc_shortReads
v48 v49 218 218 }}} 219 219 220 If you get an error like "Invalid quality score value" , your fastq file probably has Sanger (offset 33) instead of Illumina (ASCII offset 64) quality scores.220 If you get an error like "Invalid quality score value" or segmentation fault, your fastq file probably has Sanger (offset 33) instead of Illumina (ASCII offset 64) quality scores. 221 221 You'll need to add the option "-Q33" to your FASTX Toolkit arguments. 222 222